LOCUS       sequence-18381-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V956 pLPS-3'ECFP - Acceptor
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..48)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(258..260,261..974)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGVDELYK"
     primer_bind     complement(300..321)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(561..580)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     908..929
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcataact tcgtatagca tacattatac gaagttatag atccaatatt
       61 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtggatc
      181 tcgagctcaa gcttcgaatt cagggtttcc ttgacaatat catacttatc ctgtcccttt
      241 tttttccaca gctaccggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      301 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      361 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      421 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
      481 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      541 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      601 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      661 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
      721 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
      781 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      841 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      901 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcgtagacg
      961 agctgtacaa gtaatctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     1021 tttaaaaacc tcccacacct ccccctgaac c
//
