LOCUS       sequence-183822-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter - constitutive expression of mVenus 
            (515/528) localized in the nucleus, mCherry (587/610) untargeted and
            visualized in the cytoplasm, and Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Calderon GA, Thai P, Hsu CW, Grigoryan B, Gibson SM, Dickinson ME, 
            Miller JS
  TITLE     Tubulogenesis of co-cultured human iPS-derived endothelial cells and
            human mesenchymal stem cells in fibrin and gelatin methacrylate 
            gels.
  JOURNAL   Biomater Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h.
  PUBMED    28661522
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomater 
            Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h."
COMMENT     Sequence Label: hEF1-alpha-H2B-mVenus-IRES-mCherry PGK-Puromycin
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    165..213
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     238..257
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(315..914)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(398..418)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     895..914
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(926..1425)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(974..993)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1367..1390
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        1468..2637
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1706..2628
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2585..2605
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(3304..3315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3842..4393
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3986..4003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4213..4232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5969..6557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6022..6042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6440..6451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6650..6883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        6920..7011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7012..7821
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7079..7098)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7907..8495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8396..8415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8633..8859
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8860..9040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9087..9212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9705..9938
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10123..10167
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
       61 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
      121 atcgatcacg agactagcct cgagatcccg aaccccgcgt ttatgaacaa acgacccaac
      181 acccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
      241 tctccttccg tgtttcagtt agcctccccc atctcccggt cgagaagctt acgcgtgaat
      301 ttcgacgcgg ccgctcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
      361 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
      421 ggcgcggatg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
      481 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgacacgcc gacggtggcc
      541 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
      601 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
      661 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
      721 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
      781 cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
      841 gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc
      901 ttgtactcgg tcattgtggc ggcgacgaaa ggcccggaga tgaggaagag gagaacagcg
      961 cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc
     1021 ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct
     1081 gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct
     1141 tccattgctc agcggtgctg tccatctgca cgagactagt gagtcgtgct acttccattt
     1201 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     1261 tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg
     1321 tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta
     1381 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag agaaacttga
     1441 tctgtcgccg caattcaagc ttcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
     1501 tcgcccacag tccccgagaa gttgggggga ggctctggct gcaggtaatt gaaccggtgc
     1561 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     1621 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     1681 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     1741 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctcc agtacgtgat
     1801 tcttgatccc gagctggagc caggggcggg ccttgcgctt taggagcccc ttcgcctcgt
     1861 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     1921 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacgtgct
     1981 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaggatct gcacactggt
     2041 atttcggttt ttgggcccgc ggccggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2101 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2161 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2221 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctcca
     2281 gggggctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     2341 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     2401 ccgtccaggc acctcgatta gttctggagc ttttggagta cgtcgtcttt aggttggggg
     2461 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     2521 gcttggcact tgatgtaatt ctccttggaa tttggccttt ttgagtttgg atcttggttc
     2581 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaca
     2641 cctgcgaacg gggtgagacg acacccaagc tgtctagagc cgccaccatg cccgagcctg
     2701 cgaagtcagc cccagcacct aagaagggaa gtaagaaggc cgtcaccaaa gcgcagaaga
     2761 agggagggaa gaagagaaag cggagccgga aggaaagcta ctccatctac gtgtacaaag
     2821 tgctgaaaca agtgcatccg gacactggta tcagctccaa ggccatgggc atcatgaact
     2881 cctttgtgaa cgatatcttc gagcgcattg ccggagaggc ttcccgcctg gcccactaca
     2941 acaagcgcag caccataacc agccgcgaaa ttcagaccgc cgtgcgactc ctgctgcccg
     3001 gagaactggc caagcacgcc gtgtccgagg gcactaaggc cgtgaccaag tacacttcag
     3061 caaaggaccc cgtctgcgag cacccgggaa tctgtgggtc gaccatggtg tcgaagggag
     3121 aggagctgtt caccggggtg gtgccgattc tggtggaact tgatggcgac gtcaatggac
     3181 acaagttctc agtgtccggg gagggagaag gagatgctac ctatgggaag ctgactctga
     3241 agctcatctg cacaacggga aagctgccgg tgccgtggcc tactctggtc accaccttgg
     3301 gttacggcct tcaatgcttc gcgcggtacc cagaccacat gaagcaacat gacttcttta
     3361 agtccgccat gcccgaagga tacgtgcagg aaagaactat cttcttcaag gatgacggaa
     3421 actacaagac cagggctgaa gtgaaattcg agggagacac tctcgtgaac cggatcgaac
     3481 tgaaggggat cgactttaag gaagatggca acattctggg gcacaagctg gaatacaatt
     3541 acaactcgca taacgtctac atcaccgcgg acaagcagaa gaacgggatc aaggccaact
     3601 tcaagattag gcacaacatc gaggacggcg gagtgcagtt ggccgatcac taccagcaga
     3661 ataccccaat cggagatggc cccgtgttgc tccctgacaa ccattacctg tcgtatcagt
     3721 ccaagctgtc caaggacccc aacgagaagc gggaccacat ggtgctcctg gagttcgtga
     3781 ccgccgccgg catcaccctg ggcatggacg agctttacaa atgagaattc ccccccccct
     3841 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3901 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3961 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     4021 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     4081 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4141 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4201 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4261 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     4321 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     4381 cacgatgata atatgctagc ggccacaacc atggtgtcaa agggggagga ggataatatg
     4441 gcgattatca aggagtttat gaggttcaaa gtgcacatgg aaggcagcgt gaacggccac
     4501 gagttcgaaa tcgaaggaga aggggagggt cggccttatg aggggaccca gactgctaag
     4561 ctcaaggtca ccaagggcgg ccccctgccg tttgcctggg acatcctgtc cccccaattc
     4621 atgtacggtt ccaaggccta cgtgaagcac ccagcagata ttcctgacta cctgaagctc
     4681 tcgttcccgg aaggattcaa atgggagaga gtgatgaact tcgaagatgg aggcgtcgtg
     4741 acagtgaccc aggacagcag cctgcaggac ggggagttca tctacaaagt caagttgcgg
     4801 gggaccaact tcccgtccga cggccccgtg atgcaaaaga aaactatggg atgggaggcc
     4861 tcgtccgaac ggatgtaccc ggaggacgga gccctgaagg gagaaatcaa gcagcgcctg
     4921 aagctgaagg acggaggtca ttacgacgcc gaagtcaaga cgacttacaa ggctaagaag
     4981 cccgtgcagc ttcctggagc gtacaacgtg aacatcaagc tggatattac ctcccacaac
     5041 gaagattaca ccatcgtgga gcagtacgaa agagccgagg gacgccattc caccggcgga
     5101 atggacgaac tctacaaata gggatcccgt ctctaaaact gttctcatca catcatatca
     5161 aggttatata ccatcaatat tgccacagat gttacttagc cttttaatat ttctctaatt
     5221 tagtgtatat gcaatgatag ttctctgatt tctgagattg agtttctcat gtgtaatgat
     5281 tatttagagt ttctctttca tctgttcaaa tttttgtcta gttttatttt ttactgattt
     5341 gtaagacttc tttttataat ctgcatatta caattctctt tactggggtg ttgcaaatat
     5401 tttctgtcat tctatggcct gacttttctt aatggttttt taattttaaa aataagtctt
     5461 aatattcatg caatctaatt aacaatcttt tctttgtggt taggactttg agtcataaga
     5521 aatttttctc tacactgaag tcatgatggc atgcttctat attattttct aaaagattta
     5581 aagttttgcc ttctccattt agacttataa ttcactggaa tttttttgtg tgtatggtat
     5641 gacatatggg ttccctttta ttttttacat ataaatatat ttccctgttt ttctaaaaaa
     5701 gaaaaagatc atcattttcc cattgtaaaa tgccatattt ttttcatagg tcacttacat
     5761 atatcaatgg gtctgtttct gagctctact ctattttatc agcctcactg tctatcccca
     5821 cacatctcat gctttgctct aaatcttgat atttagtgga acattctttc ccattttgtt
     5881 ctacaagaat atttttgtta ttgtctttgg gctttctata tacattttga aatgaggttg
     5941 acaagttagc gtgacgtcaa ttcccgataa tcaacctctg gattacaaaa tttgtgaaag
     6001 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6061 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6121 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6181 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6241 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6301 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6361 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6421 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6481 gccggctctg cggcctcttc cgcctcttcg ccttcgccct cagacgagtc ggatctccct
     6541 ttgggccgcc tccccgcatc gggaattgcg cacgcagtgg tacctttaag accaatgact
     6601 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6661 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcatcagaat tggttaattg
     6901 gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagccat
     7021 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     7081 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     7141 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     7201 acagatgaga tggtcagact aaactggctg acggaattta tgccacttcc gaccatcaag
     7261 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     7321 gcgttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     7381 gtgttcctgc gccggttgca ctcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     7441 gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     7501 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     7561 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     7621 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     7681 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     7741 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     7801 atgctcgatg agtttttcta actcatgacc aaaatccctt aacgtgagtt acgcgcgcgt
     7861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8041 taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag
     8101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcagctt aatgtagtct
     8641 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     8701 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     8761 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     8821 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     8881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     9061 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     9121 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     9181 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     9241 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     9301 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     9361 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     9421 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     9481 aagacaccaa ggaagcttta gacaagatag aggaacagca aaacaaaagt aagaccaccg
     9541 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9601 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9661 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9721 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9781 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9841 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9901 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9961 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10021 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    10081 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    10141 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    10201 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    10261 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    10321 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    10381 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt a
//
