LOCUS       sequence-183825-       11545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11545)
  TITLE     Jing Wang lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUAST_mLExA-VP16-NFATc1 (C-del)
FEATURES             Location/Qualifiers
     source          1..11545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..95
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        114..352
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     299..320
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(395..646,647..1000)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLEKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     938..959
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             1046..1279
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     intron          3136..3201
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3331..3351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3623..3757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3660..3679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3714..3733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3784..3802)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4254..4325,4727..5000,5075..5729,5791..6106,6310..6441,
                     6512..7126)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7905..8459,8460..8490)
                     /label=P element 5' end
     rep_origin      complement(8725..9313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8805..8824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9484..10275,10276..10344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10107..10126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10345..10449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10517..10535
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10573..10595)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10695..10714
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(11252..11282,11283..11484)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
       61 agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagcga gcgccggagt
      121 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
      181 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca
      241 gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc
      301 aactactgaa atctgccaag aagtaattat tgaatacaag aagagaactc tgaataggga
      361 attgggaatt cgttaacaga tctgcggccg cggaatgaaa gcgttaacgg ccaggcaaca
      421 agaggtgttt gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc
      481 ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc
      541 gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt
      601 gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct
      661 ggcgcaacag catattgaag gtcattatca ggtcgatcct tccttattca agccgaatgc
      721 tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga
      781 cttgctggca gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat
      841 tgatgacgag gttaccgtta agggcctgga aaaacagggc aataaagtcg aactgttgcc
      901 agaaaatagc gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga
      961 agggctggcg gttggggtta ttcgcaacgg cgactggctg gaattcccgg ggatccgtcg
     1021 acctgcagga attcccgggg atctggcccc cccgaccgat gtcagcctgg gggacgagct
     1081 ccacttagac ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct
     1141 ggacatgttg ggggacgggg attccccggg tccgggattt accccccacg actccgcccc
     1201 ctacggcgct ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccttgg
     1261 aattgacgag tacggtgggt ctagaatgcc aagcaccagc tttccagtcc cttccaagtt
     1321 tccacttggc cctgcggctg cggtcttcgg gagaggagaa actttggggc ccgcgccgcg
     1381 cgccggcggc accatgaagt cagcggagga agaacactat ggctatgcat cctccaacgt
     1441 cagccccgcc ctgccgctcc ccacggcgca ctccaccctg ccggccccgt gccacaacct
     1501 tcagacctcc acaccgggca tcatcccgcc ggcggatcgc ccctcggggt gcggaacagc
     1561 tttggacggt gggcccgcgg gctacttcct ctcctccggc cacaccaggc ctgatggggc
     1621 ccctgccctg gagagtcctc gcatcgagat aacctcgtgc ttgggcctgt accacaacaa
     1681 taaccagttt ttccacgatg tggaggtgga agacgtcctc cctagctcca aacggtcccc
     1741 ctccacggcc acgctgagtc tgcccagcct ggaggcctac agagacccct cgtgcctgag
     1801 cccggccagc agcctgtcct cccggagctg caactcagag gcctcctcct acgagtccaa
     1861 ctactcgtac ccgtacgcgt ccccccagac gtcgccatgg cagtctccct gcgtgtctcc
     1921 caagaccacg gaccccgagg agggctttcc ccgcgggctg ggggcctgca cactgctggg
     1981 ttccccgcag cactccccct ccacctcgcc ccgcgccagc gtcactgagg agagctggct
     2041 gggtgcccgc tcctccagac ccgcgtcccc ttgcaacaag aggaagtaca gcctcaacgg
     2101 ccggcagccg ccctactcac cccaccactc gcccacgccg tccccgcacg gctccccgcg
     2161 ggtcagcgtg accgacgact cgtggttggg caacaccacc cagtacacca gctcggccat
     2221 cgtggccgcc atcaacgcgc tgaccaccga cagcagcctg gacctgggag atggcgtccc
     2281 tgtcaagtcc cgcaagacca ccctggagca gccgccctca gtggcgctca aggtggagcc
     2341 cgtcggggag gacctgggca gccccccgcc cccggccgac ttcgcgcccg aagactactc
     2401 ctctttccag cacatcagga agggcggctt ctgcgaccag tacctggcgg tgccgcagca
     2461 cccctaccag tgggcgaagc ccaagcccct gtcccctacg tcctacatga gcccgaccct
     2521 gcccgccctg gactggcagc tgccgtccca ctcaggcccg tatgagcttc ggattgaggt
     2581 gcagcccaag tcccaccacc gagcccacta cgagacggag ggcagccggg gggccgtgaa
     2641 ggcgtcggcc ggaggacacc ccatcgtgca gctgcatggc tacttggaga atgagccgct
     2701 gatgctgcag cttttcattg ggacggcgga cgaccgcctg ctgcgcccgc acgccttcta
     2761 ccaggtgcac cgcatcacag ggaagaccgt gtccaccacc agccacgagg ctatcctctc
     2821 caacaccaaa gtcctggaga tcccactcct gccggagaac agcatgcgag ccgtcattga
     2881 ctgtgccgga atcctgaaac tcagaaactc cgacattgaa cttcggaaag gagagacgga
     2941 catcgggagg aagaacacac gggtacggct ggtgttccgc gttcacgtcc cgcaacccag
     3001 cggacgcacg ctgtccctgc aggtggcctc caaccccatc gaatgctcct gaactagagg
     3061 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     3121 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     3181 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     3241 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     3301 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     3361 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     3421 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     3481 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     3541 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3601 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3721 gtccaaactc atcaatgtat cttatcatgt ctggatccac tagaaggcct aattctagta
     3781 tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt
     3841 tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact tcgatccaag
     3901 ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat tccactcaaa
     3961 gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt tcgccgctgc
     4021 gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt
     4081 tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc aattaaacac
     4141 aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg agtgattggg
     4201 gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc
     4261 aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc gagcatctga
     4321 acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag gaactgcgat
     4381 tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat
     4441 tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg ctatataaga
     4501 caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc gactatattc
     4561 ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc acaaaaaata
     4621 aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc ggggttagat
     4681 gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc
     4741 ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctccg
     4801 gccacccagt ccgccggagg actccggttc agggagcggc caactagccg agaacctcac
     4861 ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg
     4921 gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag
     4981 gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct
     5041 taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa
     5101 cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc
     5161 tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa
     5221 cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt
     5281 atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg gatgccacga
     5341 catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc
     5401 agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga
     5461 gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct tctgatctgc
     5521 gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca ggtgctgaag
     5581 aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg
     5641 tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact
     5701 cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta
     5761 gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa
     5821 tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg
     5881 tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc gggatatgga
     5941 gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga atgggtacac
     6001 ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt
     6061 gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag
     6121 tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagatag
     6181 aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt
     6241 gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga gtaacatttt
     6301 aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa
     6361 gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa
     6421 aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt tcaataattt
     6481 actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat
     6541 gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc
     6601 cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat
     6661 cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc
     6721 caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc
     6781 gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa
     6841 ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc
     6901 caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac
     6961 atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc
     7021 cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt
     7081 gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg
     7141 aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg tttattgccc
     7201 cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat
     7261 acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa
     7321 agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg
     7381 gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac
     7441 cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca
     7501 ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa
     7561 atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca
     7621 atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta
     7681 atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt
     7741 tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg
     7801 ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt
     7861 aatttagaaa atgcttggat ttcactggaa ctagaattaa ttcggctgct gctctaaacg
     7921 acgcatttcg tactccaaag tacgaatttt ttccctcaag ctcttatttt cattaaacaa
     7981 tgaacaggac ctaacgcaca gtcacgttat tgtttacata aatgattttt tttactattc
     8041 aaacttactc tgtttgtgta ctcccactgg tatagccttc ttttatcttt tctggttcag
     8101 gctctatcac tttactaggt acggcatctg cgttgagtcg cctcctttta aatgtctgac
     8161 cttttgcagg tgcagccttc cactgcgaat cattaaagtg ggtatcacaa atttgggagt
     8221 tttcaccaag gctgcaccca aggctctgct cccacaattt tctcttaata gcacacttcg
     8281 gcacgtgaat taattttact ccagtcacag ctttgcagca aaatttgcaa tatttcattt
     8341 ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt
     8401 cctctcaaca agcaaacgtg cactgaattt aagtgtatac ttcggtaagc ttcggctatc
     8461 gacgggacca ccttatgtta tttcatcatg ggccagaccc acgtagtcca gcggcagatc
     8521 ggcggcggag aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt
     8581 cgacgatgtg cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc
     8641 ctcgttggcg taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga
     8701 gcccgagttc aagaagaagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9781 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10081 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10441 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10501 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10561 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10621 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10681 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10741 aataccgcac cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg
    10801 tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg
    10861 gtggaggtga attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc
    10921 ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct
    10981 tttggttaga agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt
    11041 attctatagg tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg
    11101 gggcaataaa cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa gcagttattt
    11161 cggatatatg tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc
    11221 acccggaagc tcacgatgag aatggccaga ccatgatgaa ataacataag gtggtcccgt
    11281 cggcaagaga catccactta acgtatgctt gcaataagtg cgagtgaaag gaatagtatt
    11341 ctgagtgtcg tattgagtct gagtgagaca gcgatatgat tgttgattaa cccttagcat
    11401 gtccgtgggg tttgaattaa ctcataatat taattagacg aaattatttt taaagtttta
    11461 tttttaataa tttgcgagta cgcaaagctt ggctgcatcc aacgcgttgg gagctctccg
    11521 gatccaagct tgcatgcctg caggt
//
