LOCUS       sequence-183828-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             2605..2613
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     intron          3647..4219
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4293..4302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4299..4301,4302..4304,4305..5018)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4299..4301,4302..4304,4305..5015)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4344..4365)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4605..4624)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4952..4973
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5063..5197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5087..5106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5141..5160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5634..5821,5822..5833)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5867..5883)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5867..5883)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5920..5940)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5920..5938)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5959..5975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5959..5975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5972..5994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5983..5999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6007..6013,6014..6031,6032..6037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6052..6073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6190..6207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6361..6949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6441..6460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7120..7911,7912..7980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7743..7762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7981..8085)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8111..8566)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8248..8269)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8460..8479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8693..8715
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8707..8724
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8708..8724
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8734..8753
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8734..8752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10604..10615,10616..10778)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctga aagaggtact cccaagggtt
     1741 ttctggtccc atagccctca tccacagagc ctttcagagc tgtgagtccc atggctctgt
     1801 tcctgtagaa ggatctcatt gggactggcc ctgtgagtgc ttcccacaga cttcctgcag
     1861 agcctggctg agccagctac tgtgggagct ggctggtctt tgggggttct tggagtgcct
     1921 ccttggagga acaaggggct ctcagcagga agctggaagg gatgctgcgt ttagggagag
     1981 cgagggtgga gcccagatta ttgacctgta tggaggtgca aatgagggat gctcaagaga
     2041 gaaccacaga gaatggcttc tgcagagacc aggatctcct aagagatttc attgtctgcc
     2101 agagacaggg agggcagccc tgtttcctcc attccctccg ctccttctcc ttttcctctt
     2161 cctcctcctc tccttcctcc tcttcctcct cctctatctc ttagaccctg aaagctacta
     2221 gcaagtgggg tggaggtgtt cctatgagag actgacaaag ctttttatgt ccacatccct
     2281 agagaccagt gccccccctc cccacccaac tccatacttt aataggggca ggagaggatt
     2341 catctctgaa ggaagatgaa agctgtcact tgctatcttt gactgatgtt tggactccct
     2401 ggggaacagt ggattttagg tccaacttcc cctgcccagc acatggattg ggagtgagtg
     2461 tggaccagct aaaacaaaag ggttgtactg ggtcacatcc caggctgtgg gggtgggggt
     2521 ggggctggtg tcacagagcc tcaggctttg tcaaatgagg ccgatggatc ttcacatttc
     2581 ctacctgtgg cccctaaaag cgggacctcc ttatcctcaa gctgaagaat atttactttt
     2641 tttttttttt ttaaacttcc tatctgtctg gaaatgacag agatcagcgg caacagaaag
     2701 aggactcctt gttccagtgc agaacaccca cacccttcga actcttcacc cagccttggc
     2761 ctgacttcac ccgggagtgg gagagggagg ggacagggag gctgggccct ggccggagga
     2821 caagaagctc ctctgcctgg gaacaaaagc tttttttttt ttttttcttt ctggaaagct
     2881 tggtgcaagt tgggggagat gggggaggga aggcaggagg gaacgtgggg acagttctaa
     2941 tgaaaatgtc cccagggtga tagagctgtg atctgagagt cctttaaaag tctggttagc
     3001 tattgaaaaa cacttggctg gcccctagtc ttccactctc cctgtccttg catcaagctg
     3061 gggactgact ctatccttaa agaggtaggc tttacagcct ggaatgtggt cagctggttg
     3121 actgccgccc gctgtgccag aagccctttg tttgactccc aggattatta tgtaaactgg
     3181 atgtaatggt acatgctaat cccaacatcc aggaggtggt ggaggcgacc gagcattcga
     3241 ggtcatcctt ggctacgtag tgaattccag gttagcctga gttacatgag cccctgtctc
     3301 tcaaagacaa ccaaaccaac caagtaagca aacaaagaag agaccgggct ggggagagaa
     3361 ctcaatgttg tcttgcacag cacctgaacc cagtgggaaa aaccaaagct gggtgtgtgc
     3421 tcagggatgc agagcgagag accggggatc cctagggtcc acttgtcagt cagctccagg
     3481 tcccagtgga agactttgtc tcaaaaaaca aggtagatgg ctcctgagga gagatatggt
     3541 aggttgagat ctctcgactc tagagggtat ataatggatc ccatcgcgtc tcagcctcac
     3601 tttgagctcc tccacacgaa tttcgaccga tcctgagaac ttcagggtga gtttggggac
     3661 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
     3721 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
     3781 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
     3841 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
     3901 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
     3961 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     4021 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     4081 caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     4141 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     4201 cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     4261 tggcaaagaa ttcctcgacg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     4321 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4381 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     4441 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     4501 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4561 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4621 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4681 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4741 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4801 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4861 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4921 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4981 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgccac cgcggtggag
     5041 ctcgaattaa ttcatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     5101 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5161 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5221 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     5281 atgtggtatg gctgattatg atcctctaga tcagatccga agatacggcc acgggtgctc
     5341 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     5401 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     5461 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     5521 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     5581 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     5641 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     5701 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     5761 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     5821 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     5881 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     5941 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6001 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6121 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8101 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8161 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     8221 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     8281 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     8341 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     8401 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     8461 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     8521 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     8581 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8641 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     8701 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     8761 ggtaccaaat agtaggaatt acccacctgt acaagtgctg aaaacttgga tgaataagcc
     8821 cgtttgccat tttctactgc tattttaaat cttttctgta tttgtctgca tttgtcttta
     8881 cccttgaata aatgttttag ttgttttttt tcaattatga ctgtgtttaa cagacattat
     8941 tacaataata tgtaatagta gtacacacta ttattatgta atagtacttg ttgactgtat
     9001 ttgagaactg gaccgagtga gtgttacgtc acccattcaa aatgacttac ttctggctcc
     9061 aacaaaatga agttaattca gttgccattt ttcactgtat ggacatcgcc gtgttggagc
     9121 tagacgttgc caataagcaa gaaagagccg agatgcgtcg agtctgagtc accgttccta
     9181 tggcaacccc tctaaccaat cagaagtaag cttgttggaa gtccacagcc taccacttga
     9241 aagcgggctg cacaaaatct gtcaaacgtt ttgaacgttg gatgtgagag cacatacttt
     9301 tattaaggca tctggttggt cagtttataa tatcaacaac ttgggctaca gaaaagaaaa
     9361 gttattacag aaattatgat taacaagtac atgttaaata aagattttaa tatgaatgcc
     9421 accactggag cattcatgcc atttggagct tcttcctgtt tggatcacta gaaggaggag
     9481 gtcactcatt acagttctca tatacagtcg ttggttggtt ggttggttgg tagattgatt
     9541 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     9601 gattgattgg tagtcaaaat aagaaataat ttccacagat tcattacaga aatgattaaa
     9661 tgcatacata aaaaactggg gggggggggg natacaacaa cacacttaag tcacatttgc
     9721 ctacgtaaag aaaagtaaag aaaatcaata gctatatttt acatctcctt tttttgctgt
     9781 cttttaatta gccttgtttt gctgttatct tgattgaaac tgtaacttct tcacctgctt
     9841 cttttctttg taggttttgc cagcccagaa acaggcatgg ctgtgcaagg ccagagcacc
     9901 atgcacaatg cgctgcatgt cttcatgaac ggctcaatgt cctcagtcca gggctcagcc
     9961 aacgacccca tcttcctcct tcaccatgct ttcattgaca ggtaacaaac acgtcatgac
    10021 attagactgc acagtttttg acaaagttca tacaatctgt tgtttatagc tgctacaatt
    10081 agtgaagttt gtgaatgtac ttggatgagc agcgaaagat caattgagat caattgttag
    10141 agtttggttg ccctgcagag caaagaacaa aaaataatct ggtggcttta ctgcgtgagg
    10201 ttattattgg tggaatagaa acacaaaaca taattgcatt tatttgttta attttttatc
    10261 ttatcttaac tttcatcttg catatttgtt tcttacatca tttctagcat ctttgagcgc
    10321 tggctaagaa ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc
    10381 cacaatgacg gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc
    10441 ctgtccaaca atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca
    10501 acaccagaaa tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc
    10561 actctgttcc acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta
    10621 ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa
    10681 gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt
    10741 gtacttgagt attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt
    10801 aagcttttaa tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga
    10861 actgtttgta tttaattatc aaactataag acagacaatc taatgccagt acacgctact
    10921 caaagttgta aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt
    10981 caagcctagc tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg
    11041 cagccgatgc gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg
    11101 tgagcagaga ctccctggtg tctgaaacac aggccagatg ggccctcgag gatatc
//
