LOCUS       sequence-183829-        6822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW,
            Vogelstein B, Clevers H
  TITLE     Constitutive transcriptional activation by a beta-catenin-Tcf 
            complex in APC-/- colon carcinoma.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1784-7.
  PUBMED    9065401
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1784-7."
COMMENT     Sequence Label: pcDNA/Myc DeltaN TCF4
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    343..477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(380..399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     434..453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1328..1463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1958..1977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(2090..2548)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2227..2248)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2442..2461
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(2672..2690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2758..2862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2863..2931,2932..3723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3081..3100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        3796..4175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4176..4379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4330..4350
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4424..4443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4424..4442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           4457..4499
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      4521..4530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4530..4559
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     3'UTR           6678..6803
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
       61 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
      121 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
      181 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc
      241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      301 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat
      481 cccgccatgg tatcaacgcc atatttctat ttacagtagg gacctcttcg ttgtgtaggt
      541 accgctgtat tcctagggaa atagtagagg caccttgaac tgtctgcatc agccatatag
      601 cccccgctgt tcgatttaca aacacaggca cagtactgac aaacccatac acctcctctg
      661 aaatacccat agttgctagg gctgtctccg aactcattac accctccaaa gtcagagctg
      721 taatttcgcc atcaagggca gcgagggctt ctccagataa aatagcttct gccgagagtc
      781 ccgtaagggt agacacttca gctaatccct cgatgaggtc tactagaata gtcagtgcgg
      841 ctcccatttt gaaaattcac ttacttgatc agcttcagaa gatggcggag ggcctccaac
      901 acagtaattt tcctcccgac tcttaaaata gaaaatgtca agtcagttaa gcaggaagtg
      961 actaactgac cgcagctggc cgtgcgacat cctcttttaa ttagttgcta ggcaactgcc
     1021 ctccagaggg cagtgtggtt ttgcaagagg aagcaaaaag cctctccacc caggcctaga
     1081 atgtttccac ccaatcatta ctatgacaac agctgttttt tttagtatta agcagaggcc
     1141 gggggcccct ggcctccgct tactctggag aaaaagaaga gaggcattgt agaggcttcc
     1201 agaggcaact tgtcaaaaca ggactgcttc tatttctgtc acactgtctg gccctgtcac
     1261 aaggtccagc acctccatac cccctttaat aagcagtttg ggaacgggtg cgggtcttac
     1321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     1381 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     1441 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaattcggc gtaatctgct
     1501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1681 ctctgctgaa gccagttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1741 ttggactcaa gagatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2041 ggcggagcct atggaaaaac gccagcaacg caagctagct tctagctaga aattgtaaac
     2101 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     2161 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt
     2221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     2281 cgaaaaaccg tctatcaggg cgatggccgc ccactacgtg aaccatcacc caaatcaagt
     2341 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2401 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2461 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2521 gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgagca cgtataacgt
     2581 gctttcctcg ttagaatcag agcgggagct aaacaggagg ccgattaaag ggattttaga
     2641 caggaacggt acgccagctg gacccgaaag ggcctcgtga tacgcctatt tttataggtt
     2701 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     2761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2941 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3001 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3061 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     3121 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3181 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3241 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3301 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3361 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     3421 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3481 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3541 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3601 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3661 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3721 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3781 tttctctagc gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     3841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4021 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     4201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4321 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4381 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
     4441 gggagaccca agcttgcttg ttctttttgc agaagctcag aataaacgct caactttggc
     4501 agatctgatc tagaactagt gccgccatgg agcagaagct gatcagcgag gaggacctgc
     4561 agcccgggga tcccgaaaac tcctcggcag agagggattt agctgatgtc aaatcgtctc
     4621 tagtcaatga atcagaaacg aatcaaaaca gctcctccga ttccgaggcg gaaagacggc
     4681 ctccgcctcg ctccgaaagt ttccgagaca aatcccggga aagtttggaa gaagcggcca
     4741 agaggcaaga tggagggctc tttaaggggc caccgtatcc cggctacccc ttcatcatga
     4801 tccccgacct gacgagcccc tacctcccca acggatcgct ctcgcccacc gcccgaacct
     4861 atctccagat gaaatggcca ctgcttgatg tccaggcagg gagcctccag agtagacaag
     4921 ccctcaagga tgcccggtcc ccatcaccgg cacacattgt ctctaacaaa gtgccagtgg
     4981 tgcagcaccc tcaccatgtc caccccctca cgcctcttat cacgtacagc aatgaacact
     5041 tcacgccggg aaacccacct ccacacttac cagccgacgt agaccccaaa acaggaatcc
     5101 cacggcctcc gcaccctcca gatatatccc cgtattaccc actatcgcct ggcaccgtag
     5161 gacaaatccc ccatccgcta ggatggttag taccacagca aggtcaacca gtgtacccaa
     5221 tcacgacagg aggattcaga cacccctacc ccacagctct gaccgtcaat gcttccatgt
     5281 ccaggttccc tccccatatg gtcccaccac atcatacgct acacacgacg ggcattccgc
     5341 atccggccat agtcacacca acagtcaaac aggaatcgtc ccagagtgat gtcggctcac
     5401 tccatagttc aaagcatcag gactccaaaa aggaagaaga aaagaagaag ccccacataa
     5461 agaaacctct taatgcattc atgttgtata tgaaggaaat gagagcaaag gtcgtagctg
     5521 agtgcacgtt gaaagaaagc gcggccatca accagatcct tgggcggagg tggcatgcac
     5581 tgtccagaga agagcaagcg aaatactacg agctggcccg gaaggagcga cagcttcata
     5641 tgcaactgta ccccggctgg tccgcgcggg ataactatgg aaagaagaag aagaggaaaa
     5701 gggacaagca gccgggagag accaatgaac acagcgaatg tttcctaaat ccttgccttt
     5761 cacttcctcc gattacagac ctgagcgctc ctaagaaatg ccgagcgcgc tttggccttg
     5821 atcaacagaa taactggtgc ggcccttgca ggagaaaaaa aaagtgcgtt cgctacatac
     5881 aaggtgaagg cagctgcctc agcccaccct cttcagatgg aagcttacta gattcgcctc
     5941 ccccctcccc gaacctgcta ggctcccctc cccgagacgc caagtcacag actgagcaga
     6001 cccagcctct gtcgctgtcc ctgaagcccg accccctggc ccacctgtcc atgatgcctc
     6061 cgccacccgc cctcctgctc gctgaggcca cccacaaggc ctccgccctc tgtcccaacg
     6121 gggccctgga cctgccccca gccgctttgc agcctgccgc cccctcctca tcaattgcac
     6181 agccgtcgac ttcttcctta cattcccaca gctccctggc cgggacccag ccccagccgc
     6241 tgtcgctcgt caccaagtct ttagaatagc tttagcgtcg tgaaccccgc tgctttgttt
     6301 atggttttgt ttcacttttc ttaatttgcc ccccaccccc accttgaaag gttttgtttt
     6361 gtactctctt aattttgtgc catgtggcta cattagttga tgtttatcga gttcattggt
     6421 caatatttga cccattctta tttcaatttc tccttttaaa tatgtagatg agagaagaac
     6481 ctcatgattc taccaaaatt tttatcaaca gctgtttaaa gtctttgtag cgtttaaaaa
     6541 atatatatat atacataact gttatgtagt tcggatagct tagttttaaa agactgatta
     6601 aaaaacaaaa aaaaaaaaag cttgcgaggg atcccccggg ctgcatctag tgactgacta
     6661 ggatctggtt accactaaac cagcctcaag aacacccgaa tggagtctct aagctacata
     6721 ataccaactt acactttaca aaatgttgtc ccccaaaatg tagccattcg tatctgctcc
     6781 taataaaaag aaagtttctt cacattctaa aaaaaaaaaa aa
//
