LOCUS       sequence-18383-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V1662 pLP-RFP - Acceptor
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..698
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERIMNFEDGGV
                     VTVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYKAKKQVQLPGAYKTDIKLDIISHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(154..172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(708..741)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgaccgtcag atccgctagc cccatggcct cctccgagga cgtcatcaag gagttcatgc
       61 gcttcaaggt gaagatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
      181 ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggatcc aaggcctacg
      241 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcat catgaacttc gaggacggcg gcgtggtgac cgtgacccag gactccaccc
      361 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      421 gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgg atgtaccccg
      481 aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact
      541 acgacgccga ggtcaagacc acctacaagg ccaagaagca ggtccagctg cctggcgcct
      601 acaagaccga catcaagctg gacatcatct cccacaacga ggactacacc atcgtggaac
      661 agtacgagcg cgccgagggc cgccactcca ccggcgccca agcttcgata acttcgtata
      721 gcatacatta tacgaagtta tagatccaat attattgaag catttatcag ggttattgtc
      781 tcatg
//
