LOCUS       sequence-18384-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V1663 pLPS-3'RFP - Acceptor
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..44)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(384..402)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atccgctagc ataacttcgt atagcataca ttatacgaag ttatagatcc aatattattg
       61 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      121 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tggatctcga
      181 gctcaagctt cgaattcagg gtttccttga caatatcata cttatcctgt cccttttttt
      241 tccacagcta ccggtggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt
      301 gaagatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      361 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
      421 cgcctgggac atcctgtccc ctcagttcca gtacggatcc aaggcctacg tgaagcaccc
      481 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcat
      541 catgaacttc gaggacggcg gcgtggtgac cgtgacccag gactccaccc tgcaggacgg
      601 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtgat
      661 gcagaagaag accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc
      721 cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga
      781 ggtcaagacc acctacaagg ccaagaagca ggtccagctg cctggcgcct acaagaccga
      841 catcaagctg gacatcatct cccacaacga ggactacacc atcgtggaac agtacgagcg
      901 cgccgagggc cgccactcca ccgggcgcct aatctagatc ataatcagcc ataccacatt
      961 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1021 aatgaatgca atggtggtg
//
