LOCUS       sequence-183841-        4298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector; empty vector without insert to 
            determine baseline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase validation vector_SCP1_empty
FEATURES             Location/Qualifiers
     source          1..4298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             156..1808
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1852..1973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1863..1882)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1917..1936
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2221..2238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2392..2980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2472..2491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3180..3971,3972..4040))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4145..4193
                     /note="synthetic polyadenylation signal"
     misc_feature    4207..4298
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4247..4266
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggatctc cctagggtac ttatataagg gggtgggggc gcgttcgtcc
       61 tcagtcgcga tcgaacactc gagccgagca gacgtgccta cggaccgggc tagcgaccta
      121 aagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
      181 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
      241 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
      301 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
      361 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
      421 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
      481 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
      541 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
      601 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
      661 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
      721 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
      781 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
      841 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
      901 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
      961 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     1021 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     1081 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     1141 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     1201 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     1261 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     1321 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     1381 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     1441 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     1501 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     1561 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     1621 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     1681 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     1741 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     1801 ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     1861 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1921 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1981 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2041 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     2101 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     2161 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     2221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2821 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3121 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt
     3181 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata
     3241 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc
     3301 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac
     3361 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag
     3421 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga
     3481 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc
     3541 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca
     3601 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc
     3661 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc
     3721 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc
     3781 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc
     3841 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc
     3901 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     3961 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     4021 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt
     4081 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     4141 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     4201 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     4261 tgtccccagt gcaagtgcag gtgccagaac atttctct
//
