LOCUS       sequence-18385-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V1664 pLPS-3'RFP-mito - Acceptor
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..48)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(388..406)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcagatccgc tagcataact tcgtatagca tacattatac gaagttatag atccaatatt
       61 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtggatc
      181 tcgagctcaa gcttcgaatt cagggtttcc ttgacaatat catacttatc ctgtcccttt
      241 tttttccaca gctaccggtg gcctcctccg aggacgtcat caaggagttc atgcgcttca
      301 aggtgaagat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      421 ccttcgcctg ggacatcctg tcccctcagt tccagtacgg atccaaggcc tacgtgaagc
      481 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      541 gcatcatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc accctgcagg
      601 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      661 tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg
      721 gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg
      781 ccgaggtcaa gaccacctac aaggccaaga agcaggtcca gctgcctggc gcctacaaga
      841 ccgacatcaa gctggacatc atctcccaca acgaggacta caccatcgtg gaacagtacg
      901 agcgcgccga gggccgccac
//
