LOCUS       sequence-18386-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V25 pLP Flag - Acceptor
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(86..119)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    376..497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(413..432)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     467..486
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(641..662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggggcatta gcgagctggt tngtgaaccg tcagatccgc tagccaccat ggactacaag
       61 gacgacgcac gacaagcaag cttcgataac ttcgtatagc atacattata cgaagttata
      121 gatccaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      241 tgacgtggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
      301 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      361 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      421 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      481 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
      541 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      601 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      661 actattaaag aacgtggact ccaacgtcaa aggg
//
