LOCUS       sequence-183863-        5905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with IFIT2 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_I...
FEATURES             Location/Qualifiers
     source          1..5905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1780..1797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(1955..2023,2024..2815)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3005..3593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3085..3104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3692..3701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3698..5350
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5394..5515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5405..5424)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5459..5478
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaactagcat ttacttatta ctgccaggca ttattctaag gacatttcac gtattctctc
       61 atttcatcct cccaacacca caataaacca gtagttgtat tattcccaat ttacaaaacc
      121 gaagcagaga cattcaataa ttttccccag agtttcatag cataaaggtg gaagctgatt
      181 tcaaatccag atagacagac atcactgccc atgcttctaa tcatgtattc atttagtcaa
      241 caactacttt tggaatgctt gccataccat gcaatgtgca ggttgttttg cagggactgg
      301 agattcagca atgattcaga ctaaaatccc ctgccctgtc gaggctaaat aactgctcct
      361 tacagtggcc tgtggatcat attggtataa ggcagagcaa agatgggtta atatggtaat
      421 cacaaggcag ggagctttgt aaatgtgtcc tcctaggagt tgtgctccct tgatgccatc
      481 agtacattcc acaaagagga agttcttcta actcactacg cagctgggct tggctcttac
      541 tcaagagcat tttggggtga aaatatctaa gtttaccagc ccttgtgcaa atctgagatg
      601 aaggactaca ttaaaagtaa aattcacctt agcaggaagt ggggtttgct attctgtgta
      661 tatctttaac agtatctgct gaaaagaacc cttttgttaa ataattgtat tacaagtccc
      721 caacttaatc ttttcgaata tgaaataaga gagggacagt gcacaagagc aatgtcccca
      781 gacccatctt taagtgaagc accaggccga tgaaacatcc ctctctgctg ccttctttct
      841 ctgatcacaa ctcagctccg gaggaaaaag agtcctctaa agtataataa aaagaaaaaa
      901 agaaaaagag tcctgccaat ttcactttct agtttcactt tcccttttgt aacgtcagct
      961 gaagggaaac aaacaaaaag gaaccagagg ccacttgtat atataggtct cttcagcatt
     1021 tattggtggc agaagaggaa gatttctgaa gagtgcagct gcctgaaccg agccctgccg
     1081 aacagctgag aattgcactg caaccatgag gtaaatattt tcccttcgta ttcggtagtg
     1141 ctgttgagtc atcttgtcca atgcaaatcc tgagaagcta tgttcccaaa gagggccagc
     1201 tccattttag tgtttgttta tagccttact atgcctctac ctctgttggt tgtaaatctg
     1261 tcttaccaat ggtggtttgt tccctcctga acaattttct gcttcacact ggcaagcttc
     1321 ctaaattcat ctccagaact gcacgcctgg ggagttgagt tgcaatcaga gaaagaaggc
     1381 agcagagagg gatgatgttt catcggcctg gtgcttcact taaagatggg tctgggggca
     1441 ttgctcttgt gcactgaccc tgtcttattt catatttgaa aggattaagt tggggacttg
     1501 taatacaatt atttaacaaa agggttcttt tcagcagata ctgttaaaga tatacacaga
     1561 atagcaaacc ccacttcctg caaaggtgaa ttttactttt aatgtagttc ttcatctcag
     1621 atttgcacaa gggctggtaa acttaaatat tttcacccca aaatgcctcc tgagtatgaa
     1681 ttagagatgg agtgcttgga gaaaatcgtc gccgcactta tgactgtctt ctttatcatg
     1741 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     1801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1861 cacagaatca ggggataacg caggaaagaa catgtcttga gagacgtact agtaaccctg
     1921 ataaatgctt caataatatt gaaaaaggac gagtatgagc atccaacatt ttcgtgtcgc
     1981 actcattccc ttctttgcgg cattttgctt gcctgttttt gcacaccccg aaacgctggt
     2041 gaaagtaaaa gatgctgaag atcaactggg tgcaagagtg ggctatatcg aactggatct
     2101 caatagcggc aagatccttg agtctttccg ccccgaagaa cgattcccga tgatgagcac
     2161 tttcaaagta ctgctatgtg gcgcggtgtt gtcccgtata gacgccgggc aagagcagct
     2221 tggtcgccgt atacactact cacaaaacga cttggttgag tactcgccgg tcacggaaaa
     2281 gcatcttacg gatggcatga cggtaagaga attgtgtagt gctgccatta ccatgagcga
     2341 caacaccgcg gccaacttac ttctgacaac gatcggaggc cctaaggagc tgactgcatt
     2401 tcttcataat atgggtgatc atgtgacccg gcttgaccgc tgggaaccag agttgaacga
     2461 agccataccg aacgacgagc gtgataccac gatgccagta gcaatggcca caactcttcg
     2521 gaaactactc actggcgaac ttcttactct agcatcacga cagcagctca tagactggat
     2581 ggaggcggac aaagtagcag gaccacttct tcgctcggcc ctccctgctg gctggttcat
     2641 tgctgacaaa tcgggggccg gtgaacgcgg ctctcgcggc atcattgctg cgctggggcc
     2701 tgatggtaag ccctcacgaa tcgtagtgat ctacacgacg gggagtcagg ccactatgga
     2761 cgaacgaaat aggcagatcg ctgagatcgg tgcctcactg atcaagcact ggtaaccact
     2821 gcagtggttt agcatttgcg gccgctgtca gaccaagttt actcatatat actttagatt
     2881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2941 atgacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3121 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3181 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3241 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3301 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3361 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3421 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3481 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3541 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3601 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3661 tgaagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca aaaacattaa
     3721 gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc agctgcacaa
     3781 agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg cacatatcga
     3841 ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag aagctatgaa
     3901 gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata gcttgcagtt
     3961 cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag ctaacgacat
     4021 ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tcgtattcgt
     4081 gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa
     4141 gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt acaccttcgt
     4201 gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg
     4261 ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc ccaagggcgt
     4321 agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg
     4381 caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc acggcttcgg
     4441 catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca tgtaccgctt
     4501 cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt
     4561 gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa
     4621 cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc
     4681 caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc
     4741 cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt
     4801 cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg
     4861 cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac
     4921 aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga
     4981 ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca
     5041 ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg
     5101 ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga
     5161 acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac
     5221 cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg
     5281 caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat
     5341 cgccgtgtaa taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     5401 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5461 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5521 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     5581 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
     5641 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactaca tggtggaacc
     5701 ccatctctac taaaaataca aaaattagcc gggcgtggtg gtgcgtgcct gtaatcccag
     5761 ctactcgaga ggctgaggcg aatcacttga accagggagg tggaggttgc agtaagccag
     5821 gatctttcca ctgcactcca gcctgaaaaa aagagtttcc cctctgatca ctttggttaa
     5881 cgattttagt taataattat aatag
//
