LOCUS       sequence-183868-       13127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of human ID2 with 3xFLAG 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 13127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pSLIK TT 3xFLAG ID2 hygro
FEATURES             Location/Qualifiers
     source          1..13127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             749..929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    976..1101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1594..1827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2012..2056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2354..2471
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2528..2552
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2584..2898
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2592..2610
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2628..2646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2663..2681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2699..2717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2735..2753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2871..2895
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2907..2972
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3419..3443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3493..4704
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4541..4560
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4829..5446,5447..5452,5453..5488,5489..5491,5492..5527,
                     5528..5530,5531..5566,5567..5575)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5613..6185
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5779..5796)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6006..6025
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6244..7263
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6244..7263
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    7729..8317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7782..7802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8200..8211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8320..8336)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8321..8337)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8846..9026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9052..9069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9058..9282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9328..9756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9415..9434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9625..9646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9765..9785)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9770..10099
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9950..10085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10012..10031
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10147..10194
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10213..10587
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10717..10838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10754..10773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10808..10827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10887..10903)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10887..10903)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10900..10922)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10911..10927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10935..10941,10942..10959,10960..10965))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10980..11001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11118..11135)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11289..11877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11369..11388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12048..12839,12840..12908))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12671..12690
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12909..13013)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13088..13107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      901 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      961 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1021 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1081 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1141 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1201 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1261 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1321 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1381 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2041 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2101 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2161 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2221 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2281 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2341 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2401 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2461 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attctagaca
     2521 gttatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat
     2581 cccgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2641 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2701 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2761 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2821 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     2881 gtgaaccgtc agatcgccac tagatggact acaaagacca tgacggtgat tataaagatc
     2941 atgacatcga ttacaaggat gacgatgaca agactagtat gaaagccttc agtcccgtga
     3001 ggtccgttag gaaaaacagc ctgtcggacc acagcctggg catctcccgg agcaaaaccc
     3061 ctgtggacga cccgatgagc ctgctataca acatgaacga ctgctactcc aagctcaagg
     3121 agctggtgcc cagcatcccc cagaacaaga aggtgagcaa gatggaaatc ctgcagcacg
     3181 tcatcgacta catcttggac ctgcagatcg ccctggactc gcatcccact attgtcagcc
     3241 tgcatcacca gagacccggg cagaaccagg cgtccaggac gccgctgacc accctcaaca
     3301 cggatatcag catcctgtcc ttgcaggctt ctgaattccc ttctgagtta atgtcaaatg
     3361 acagcaaagc actgtgtggc tgacaattgg aagactaatg cgttttcgag atatctagac
     3421 ccagctttct tgtacaaagt ggttgataac atctcgagcc ataaagatgg ttaattaacc
     3481 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     3541 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     3601 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     3661 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     3721 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     3781 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     3841 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     3901 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     3961 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     4021 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     4081 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     4141 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     4201 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     4261 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     4321 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     4381 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     4441 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     4501 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     4561 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     4621 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     4681 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     4741 cggtcctgca ggatttaaat ggcgcgccgc tagcgctacc ggactcagat ctcgagctca
     4801 agcttcgaat tctgcagtcg aattcaccat gtctagactg gacaagagca aagtcataaa
     4861 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     4921 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     4981 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     5041 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     5101 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     5161 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     5221 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     5281 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     5341 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     5401 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     5461 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     5521 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     5581 taaggatccg cggccgcact agaggaattc cgcccctctc cccccccccc cctaacgtta
     5641 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5701 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5761 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5821 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5881 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5941 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6001 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6061 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6121 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6181 gataagctta ccggtacgcg tgatagcttg ccacaacccg taccaaagat ggatagatcc
     6241 ggaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     6301 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     6361 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     6421 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     6481 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     6541 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     6601 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     6661 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     6721 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     6781 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     6841 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     6901 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     6961 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     7021 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     7081 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     7141 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     7201 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     7261 tagagtagat gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg
     7321 cgagcctagc aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc
     7381 acagttcgct aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc
     7441 ttctggattg tgttggtccc cagggcacga ttgtcatgcc cacgcactcg ggtgatctga
     7501 ctgatcccgc agattggaga tcgccgcccg tgcctgccga ttgggtgcag atctctagat
     7561 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     7621 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7681 caattgttgt tgtttagtcc ctcccaattc gatatcaagc ttatcgataa tcaacctctg
     7741 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7801 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7861 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7921 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7981 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8041 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8101 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8161 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8221 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8281 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     8341 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     8401 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     8461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8521 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8581 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     8641 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     8701 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     8761 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     8821 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     8881 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8941 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9001 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     9061 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9121 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9181 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9241 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9301 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     9361 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9421 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9481 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9541 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9601 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9661 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9721 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9781 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9841 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9901 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10021 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
    10081 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
    10141 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
    10201 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    10261 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    10321 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    10381 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    10441 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    10501 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    10561 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
    10621 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    10681 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    10741 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10801 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    10861 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10921 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10981 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11041 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11101 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11221 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11281 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11341 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11401 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11461 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11521 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11581 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11641 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11701 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11761 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11821 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11881 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11941 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12001 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12061 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12121 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12181 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12241 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12301 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12361 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12421 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12481 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12541 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12601 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12661 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12721 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12781 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12841 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12901 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12961 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13021 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    13081 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctg
//
