LOCUS       sequence-18388-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V179 pLP Triple-Flag - Acceptor
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..89
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(99..132)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    389..510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(426..445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     480..499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(654..675)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     866..885
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagccac atggactaca aggaccacga cggcgactac aaggaccacg
       61 acatcgacta caaggacgac gacgacaagc aagcttcgat aacttcgtat agcatacatt
      121 atacgaagtt atagatccaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      241 gaaaagtgcc acctgacgtg gatccaccgg atctagataa ctgatcataa tcagccatac
      301 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      361 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      481 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
      541 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      601 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
      661 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
      721 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
      781 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
      841 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
      901 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
      961 taca
//
