LOCUS       sequence-18389-e         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V180 pLP Triple-Flag SD - Acceptor
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..109
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(493..526)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcagagctgg gtttagtgac cgtcagatcc gctagccacc atggactaca aggaccacga
       61 cggcgactac aaggaccacg acatcgacta caaggacgac gacgacaagg taagtagatc
      121 ttgacctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg
      181 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt
      241 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg
      301 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg
      361 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg
      421 tgcgctcgtc gggcaccagg tgcacgcgcc aacagcggtt gtgcagggtg acaaggtcaa
      481 cgctcagctt cgataacttc gtatagcata cattatacga agttatagat ccaatattat
      541 tgaagca
//
