LOCUS       sequence-18390-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V181 pLPS 3' Triple-Flag - Acceptor
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..48)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             261..326
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    440..561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(477..496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     531..550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(705..726)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcagatccgc tagcataact tcgtatagca tacattatac gaagttatag atccaatatt
       61 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtggatc
      181 tcgagctcaa gcttcgaatt cagggtttcc ttgacaatat catacttatc ctgtcccttt
      241 tttttccaca gctaccggtg gactacaagg accacgacgg cgactacaag gaccacgaca
      301 tcgactacaa ggacgacgac gacaagtaat ctagatcata atcagccata ccacatttgt
      361 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      421 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      541 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
      601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      661 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      721 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      781 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      841 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      901 acgtggcgag aaaggaaggg aa
//
