LOCUS       sequence-18391-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V143 pLP-HA SD - Acceptor
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(457..490)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcagagctgg gtttagtgac cgtcagatcc gctagccacc atgtacccat acgacgtgcc
       61 agactacgcc caggtaagta gatcttgacc tctcaaaagc gggcatgact tctgcgctaa
      121 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt
      181 tgagggtggc cgcatccatc tggtcagaaa agacaatctt tttgttgtca agcttggtgg
      241 caaacgaccc gtagagggcg ttggacagca acttggcgat ggagcgcagg gtttggtttt
      301 tgtcgcgatc ggcgcgctcc ttggccgcga tgtttagctg cacgtattcg cgcgcaacgc
      361 accgccattc gggaaagacg gtggtgcgct cgtcgggcac caggtgcacg cgccaaccgc
      421 ggttgtgcag ggtgacaagg tcaacgctaa gcttcgataa cttcgtatag catacattat
      481 acgaagttat agatccaata ttattgaagc atttatcagg gttattgtct catgagcgga
      541 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      601 aaagtgccac ctgacgtgga tccaccggat ctag
//
