LOCUS       sequence-183913-       15770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of LacZ with 3xFLAG 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 15770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pSLIK TT 3xFLAG LacZ hygro
FEATURES             Location/Qualifiers
     source          1..15770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             749..929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    976..1101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1594..1827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2012..2056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2354..2471
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2528..2552
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2584..2898
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2592..2610
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2628..2646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2663..2681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2699..2717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2735..2753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2871..2895
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2907..2972
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2979..6026
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(2981..3003)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(6062..6086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6136..7347
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7184..7203
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7472..8089,8090..8095,8096..8131,8132..8134,8135..8170,
                     8171..8173,8174..8209,8210..8218)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8256..8828
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8422..8439)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8649..8668
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8887..9906
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             8887..9906
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    10372..10960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10425..10445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10843..10854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10963..10979)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10964..10980)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11489..11669
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11695..11712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11701..11925
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11971..12399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12058..12077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12268..12289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12408..12428)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12413..12742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12593..12728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12655..12674
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12790..12837
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12856..13230
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13360..13481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13397..13416)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13451..13470
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13530..13546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13530..13546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13543..13565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13554..13570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13578..13584,13585..13602,13603..13608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13623..13644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13761..13778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13932..14520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14012..14031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14691..15482,15483..15551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15314..15333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15552..15656)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15731..15750)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      901 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      961 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1021 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1081 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1141 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1201 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1261 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1321 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1381 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2041 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2101 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2161 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2221 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2281 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2341 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2401 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2461 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attctagaca
     2521 gttatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat
     2581 cccgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2641 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2701 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2761 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2821 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     2881 gtgaaccgtc agatcgccac tagatggact acaaagacca tgacggtgat tataaagatc
     2941 atgacatcga ttacaaggat gacgatgaca agactagtgt cgttttacaa cgtcgtgact
     3001 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3061 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3121 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     3181 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     3241 atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca
     3301 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     3361 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     3421 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     3481 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt
     3541 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     3601 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     3661 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     3721 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     3781 gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     3841 aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     3901 acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     3961 ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     4021 gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     4081 tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     4141 cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     4201 aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     4261 tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     4321 ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     4381 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     4441 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     4501 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     4561 gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     4621 ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     4681 ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     4741 cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     4801 cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     4861 tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     4921 tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     4981 gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     5041 tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggc cacatcagcg
     5101 cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     5161 ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     5221 ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     5281 tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     5341 gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     5401 accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgac gcggtgctga
     5461 ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     5521 accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     5581 caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     5641 actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     5701 accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     5761 gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     5821 agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     5881 tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggag
     5941 acgactcctg gagcccgtca gtatcggcgg aattacagct gagcgccggt cgctaccatt
     6001 accagttggt ctggtgtcaa aaatgacaat tggaagacta atgcgttttc gagatatcta
     6061 gacccagctt tcttgtacaa agtggttgat aacatctcga gccataaaga tggttaatta
     6121 acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     6181 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     6241 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     6301 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     6361 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     6421 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     6481 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     6541 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     6601 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     6661 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     6721 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     6781 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     6841 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     6901 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     6961 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     7021 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     7081 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     7141 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     7201 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     7261 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     7321 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat ctccaccccg
     7381 taccggtcct gcaggattta aatggcgcgc cgctagcgct accggactca gatctcgagc
     7441 tcaagcttcg aattctgcag tcgaattcac catgtctaga ctggacaaga gcaaagtcat
     7501 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     7561 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     7621 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     7681 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     7741 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     7801 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     7861 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     7921 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     7981 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     8041 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     8101 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     8161 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     8221 aagtaaggat ccgcggccgc actagaggaa ttccgcccct ctcccccccc ccccctaacg
     8281 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     8341 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     8401 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     8461 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     8521 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     8581 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     8641 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     8701 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     8761 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     8821 gatgataagc ttaccggtac gcgtgatagc ttgccacaac ccgtaccaaa gatggataga
     8881 tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     8941 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     9001 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     9061 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     9121 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     9181 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     9241 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     9301 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     9361 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     9421 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     9481 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     9541 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     9601 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     9661 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     9721 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     9781 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     9841 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     9901 gaatagagta gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc
     9961 gcgcgagcct agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg
    10021 tccacagttc gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg
    10081 cccttctgga ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc
    10141 tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcta
    10201 gatctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
    10261 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    10321 atgcaattgt tgttgtttag tccctcccaa ttcgatatca agcttatcga taatcaacct
    10381 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    10441 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    10501 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    10561 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    10621 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    10681 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    10741 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    10801 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    10861 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    10921 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
    10981 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    11041 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
    11101 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    11161 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
    11221 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
    11281 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
    11341 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
    11401 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
    11461 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
    11521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    11581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11641 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
    11701 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    11761 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    11821 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    11881 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    11941 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    12001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    12061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    12121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    12361 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    12421 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    12481 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    12541 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    12601 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    12661 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    12721 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    12781 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
    12841 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
    12901 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
    12961 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
    13021 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
    13081 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
    13141 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
    13201 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
    13261 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    13321 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    13381 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    13441 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    13501 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    13561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    13621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    13681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    13741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    13801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    13861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    13921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    13981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    14041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    14101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    14161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    14221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    14281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    14341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    14401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    14461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    14521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    14581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    14641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    14701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    14761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    14821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    14881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    14941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    15001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    15061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    15121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    15181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    15241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    15301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    15361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    15421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    15481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    15541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    15601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    15661 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
    15721 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
//
