LOCUS       sequence-18392-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V27 pLPS-3' HA - Acceptor
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..50)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             263..289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    403..524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(440..459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     494..513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(531..988)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(668..689)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgtcagatcc gctagcataa cttcgtatag catacattat acgaagttat agatccaata
       61 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtgga
      181 tctcgagctc aagcttcgaa ttcagggttt ccttgacaat atcatactta tcctgtccct
      241 tttttttcca cagctaccgg tgtacccata cgacgtgcca gactacgcct aatctagatc
      301 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      361 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
      541 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      601 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      661 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      721 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      781 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      841 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaaagga
      901 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtacccac cacacccgcc
      961 gcgcttattg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcg
//
