LOCUS       sequence-18394-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V517 pLP-dMyc SD - Acceptor
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..57
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             64..93
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(486..519)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    776..857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(813..832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgaccgtcag atccgctagc caccatggag cagaagctga tcagcgagga ggacctgggc
       61 ggcgagcaga agctgatcag cgaggaggac ctgggcggcc aggtaagtag atcttgacct
      121 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
      181 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct ggtcagaaaa
      241 gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt tggacagcaa
      301 cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct tggccgcgat
      361 gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg tggtgcgctc
      421 gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt caacgctaag
      481 cttcgataac ttcgtatagc atacattata cgaagttata gatccaatat tattgaagca
      541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
      601 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat ccaccggatc
      661 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
      721 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
//
