LOCUS       sequence-18396-e         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V621 pGEX-4T-LP - Acceptor
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(26..59)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(267..289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(434..452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcggatctg gttccgcgtg gatccataac ttcgtatagc atacattata cgaagttata
       61 gatccaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      121 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      181 tgacgtgaat tcccgggtcg actcgagcgg ccgcatcgtg actgactgac gatctgcctc
      241 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
      301 gcttgtctgt aagcggatgc cgggagcata caagcccgtc agggcgcgtc agcgggtgtt
      361 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct
      421 tgaagacgac agggcctcgt gatacgccta tttttatagg ttaatgt
//
