LOCUS       sequence-18397-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V622 pGEX-2TK-LP - Acceptor
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    126..159
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(181..198)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(225..247)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gttaggtggc atttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      121 gatctataac ttcgtgtaat gtatgctata cgaagttatg gatccaacag atgcacgacg
      181 agatccacgc ggaaccagat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg
      241 ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg
      301 gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg
      361 gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg
      421 atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg
      481 tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga
      541 aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt
      601 tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg
      661 tgttaattta acat
//
