LOCUS       sequence-18398-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V623 pAcGHLT-LP - Acceptor
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(88..121)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catcatcatc atcacggaag gagaagggcc agtgttgcgg cgggaatttt ggtccctcgt
       61 ggaagcccag gactcgatgg catatggata acttcgtata gcatacatta tacgaagtta
      121 tagatccaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtga attcaggcct ccatgggagc tcgcggccgc ctgcagggta cccccgggag
      301 atctgtaccg actctgctga agaggaggaa attctccttg aagtttccct ggtgttcaaa
      361 gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta aaacacgata
      421 cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt tacagacaat
      481 tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta tgttagagcg
      541 aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc ctcaatagat
      601 ttgtaaaata ggtttcgatt agtttcaaac aagggttggt
//
