LOCUS       sequence-18401-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME-mating recipient 
            plasmid with a tetracycline-responsive promoter (TET) controlling 
            GFP-IRES-Neo expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-TET-GIN-recipient
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(28..602,603..605,606..614)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(195..212)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     422..441
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gctgtacagt agcggccgca aattccgccc ctctccctcc ccccccccta acgttactgg
       61 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
      121 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
      181 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
      241 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
      301 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
      361 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
      421 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
      481 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
      541 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
      601 agcttgccac aaccatggct gaa
//
