LOCUS       sequence-18405-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME-mating recipient 
            plasmid with CMV promoter controlling low-affinity nerve growth 
            factor receptor (LNGFR) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-CMV-LNGFR-recipient
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             118..298
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgctgc
      121 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
      181 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
      241 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
      301 tacagtccgg atgcagctct cgggccatgt gatgaaatgc taggcggctg tcaaacctcc
      361 actctaatac ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
      421 gggtgttctc tccttcattg gcttcttcta ccttctcttg ctcaactggt actagcttgt
      481 agcaccatcc aaaggtcagt ggatatctga tccctggccc tggtgtgtag ttctgccaat
      541 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttcgttggg
      601 agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg
      661 ccttgtaagt cattggtctt aaaggtacct gaggtgtgac tggaaaaccc acctcctcct
      721 cctcttgtgc ttctag
//
