LOCUS       sequence-18407-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with a tetracycline-responsive promoter (TET) controlling expression
            of GFP and miR30-based shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-TET-GFP-FF3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           51..172
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           199..213
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           241..367
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        521..623
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(690..709)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcggccgca agccttgtta agtgctcgct tcggcagcac atatactatg ttgaatgagg
       61 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
      121 ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgagctc
      181 ccgtgaattg gaatcctagt gaagccacag atgtaggatt ccaattcagc gggagcctgc
      241 ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta ctaaaactga
      301 ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt ataaattaaa
      361 tcactttttt caattggaag actaatgcgt ttaaacacgc ggcgacgcgt ttgaccgaat
      421 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      481 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      541 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttgtcgagat
      601 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      661 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      721 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      781 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      841 tacgtatggc aatgaaagac ggtgagctgg tga
//
