LOCUS       sequence-18408-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with CMV promoter controlling expression of GFP and miR30-based 
            shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-CMV-GFP-FF3
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           133..147
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           175..301
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        455..557
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(624..643)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcttcagg
       61 ttaacccaac agaaggctcg agaaggtata ttgctgttga cagtgagcga gctcccgtga
      121 attggaatcc tagtgaagcc acagatgtag gattccaatt cagcgggagc ctgcctactg
      181 cctcggaatt caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct
      241 tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt
      301 ttttcaattg gaagactaat gcgtttaaac acgcggcgac gcgttcgacc gaataaatac
      361 ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga
      421 agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact
      481 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttgtcg agattttcag
      541 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      601 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      661 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      721 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattacgta
      781 tggcaatgaa agacggtga
//
