LOCUS       sequence-18409-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with a CMV promoter controlling expression of dsRed and miR30-based 
            shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-CMV-dsRed-FF3
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           84..205
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           232..246
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           274..400
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        554..656
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(723..742)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcgcgccgag ggccgccacc acctgttcct gtaggcggcc gcaagccttg ttaagtgctc
       61 gcttcggcag cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct
      121 gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga
      181 gaaggtatat tgctgttgac agtgagcgag ctcccgtgaa ttggaatcct agtgaagcca
      241 cagatgtagg attccaattc agcgggagcc tgcctactgc ctcggaattc aaggggctac
      301 tttaggagca attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt
      361 ttacaaagct gaattaaaat ggtataaatt aaatcacttt tttcaattgg aagactaatg
      421 cgtttaaaca cgcggcgacg cgttcgaccg aataaatacc tgtgacggaa gatcacttcg
      481 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
      541 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
      601 cactaccggg cgtatttttt gagttgtcga gattttcagg agctaaggaa gctaaaatgg
      661 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      721 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      781 cggccttttt aaagaccgta aagaaaaata agca
//
