LOCUS       sequence-18410-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with a CMV promoter controlling expression of Neomycin and 
            miR30-based shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-CMV-Neo-FF3
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           118..239
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           266..280
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           308..434
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        588..690
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(757..776)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
       61 tcttctgagc ggccgcaagc cttgttaagt gctcgcttcg gcagcacata tactatgttg
      121 aatgaggctt cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga
      181 ttacttcttc aggttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag
      241 cgagctcccg tgaattggaa tcctagtgaa gccacagatg taggattcca attcagcggg
      301 agcctgccta ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta
      361 aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata
      421 aattaaatca cttttttcaa ttggaagact aatgcgttta aacacgcggc gacgcgttcg
      481 accgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
      541 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
      601 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagttg
      661 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      721 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      781 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      841 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctca
//
