LOCUS       sequence-18411-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with a CMV promoter controlling expression of LNGFR and miR30-based 
            shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-CMV-LNGFR-FF3
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..127)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     ncRNA           complement(281..407)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(435..449)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(476..597)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgacaactc
       61 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
      121 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
      181 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggtcgaacg
      241 cgtcgccgcg tgtttaaacg cattagtctt ccaattgaaa aaagtgattt aatttatacc
      301 attttaattc agctttgtaa aaatgtatca aagagatagc aaggtattca gttttagtaa
      361 acaagataat tgctcctaaa gtagcccctt gaattccgag gcagtaggca ggctcccgct
      421 gaattggaat cctacatctg tggcttcact aggattccaa ttcacgggag ctcgctcact
      481 gtcaacagca atataccttc tcgagccttc tgttgggtta acctgaagaa gtaatcccag
      541 caagtgtttc caagatgtgc aggcaacgat tctgtaaagt actgaagcct cattcaacat
      601 agtatatgtg ctgccgaagc gagcacttaa caaggcttgc ggccgctcac ctcttgaagg
      661 ctatgtaggc cacaagaccc acaaccacag cagccaggat ggagcaatag acagggatga
      721 ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct gcccatcact gtggtcacca
      781 cacctgccac cgtgctggct atgaggtctt gttctggagg tgcctcaggc tcctgggtgc
      841 tgggggctgt gctgtccgag ccctctgggg gtgtggaccg tgtaatccaa cggccaggga
      901 tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg gagctggcgc tcggtgtcct
      961 cgcacacggt gcagggcagg cacgggtcca cgtggttggg ctcgtcggaa tacgtgccgt
     1021 cggggcactc ctcgcacacg gtgttctgct tgtcctg
//
