LOCUS       sequence-18412-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector. pPRIME cloning plasmid 
            with a Tet-responsive promoter (TREX) controlling expression of GFP 
            and miR30-based shRNA targeting firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ
  TITLE     A lentiviral microRNA-based system for single-copy polymerase 
            II-regulated RNA interference in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.
  PUBMED    16141338
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13212-7."
COMMENT     Sequence Label: pPRIME-TREX-GFP-FF3
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           50..171
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           198..212
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           240..366
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        520..622
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(689..708)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcggccgca agccttgtta gtgctcgctt cggcagcaca tatactatgt tgaatgaggc
       61 ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct
      121 tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg agcgagctcc
      181 cgtgaattgg aatcctagtg aagccacaga tgtaggattc caattcagcg ggagcctgcc
      241 tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
      301 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
      361 cacttttttc aattggaaga ctaatgcgtt taaacacgcg gcgacgcgtt cgaccgaata
      421 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      481 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      541 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tgtcgagatt
      601 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      661 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      721 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      781 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      841 acgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      901 cgttttccca tgagcaaact gaaacgtttt catcgc
//
