LOCUS       sequence-18413-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditional gene targeting vector; contain cloning sites for 5′ and 
            3′ homology arms, and a central targeting region flanked by LoxP 
            sites, and a neomycin cassette flanked by FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     pEZ-Frt-lox-DT
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEZ-Frt-lox-DT
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(128..175)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     240..262
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     254..271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     255..271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..655)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 accctcacta aagggaaaaa agctgtcgag atctagatat cgatggccat agcggccgcg
       61 gatccataac ttcgtataat gtatgctata cgaagttatc ctttcgaggg agcttcaaaa
      121 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat
      181 ccccctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      241 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctctcccata tggtcgagca
      301 gtgtggtttt caagaggaag caaaaagcct ctccacccag gcctggaatg tttccaccca
      361 atgtcgagca gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctggaat
      421 gtttccaccc aatgtcgagc aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      481 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      541 ggcctcgaac accgagcgac cctgcagcca atatgggatc ggccattgaa caagatggat
      601 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      661 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      721 ttttttgtca gaccgacctg tccggtgccc tgaatgaact
//
