LOCUS       sequence-18417-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human ATF6 with an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Zhu C, Johansen FE, Prywes R
  TITLE     Interaction of ATF6 and serum response factor.
  JOURNAL   Mol Cell Biol. 1997 Sep . 17(9):4957-66.
  PUBMED    9271374
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1997 Sep . 17(9):4957-66."
COMMENT     Sequence Label: pCGN-ATF6
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           94..133
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac
      121 ctcttcggcc agcgccttgt agaagcgcgt atggcttcta gctatcctta tgacgtgcct
      181 gactatgcca gcctgggagg accttctaga gagcttggaa aaggaacttg tgaaatgggg
      241 gagccggctg gggttgccgg caccatggag tcacctttta gcccgggact ctttcacagg
      301 ctggatgaag attgggattc tgctctcttt gctgaactcg gttatttcac agacactgat
      361 gagctgcaat tggaagcagc aaatgagacg tatgaaaaca attttgataa tcttgatttt
      421 gatttggatt tgttaccttg ggagtcagac atttgggaca tcaacaacca aatctgtaca
      481 gttaaagata ttaaggcaga accccagcca ctttctccag cctcctcaag ttattcagtc
      541 tcatctcctc ggtcagtgga ctcttattct tcaactcagc atgttcctga ggagttggat
      601 ttgtcttcta gttctcaga
//
