LOCUS       sequence-18420-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Shinkai A, Loeb LA
  TITLE     In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) 
            in motif A functions in base selection.
  JOURNAL   J Biol Chem. 2001 Dec 14. 276(50):46759-64.
  PUBMED    11602576
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 14. 276(50):46759-64."
COMMENT     Sequence Label: pLA230
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(162..253)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(319..909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(399..418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
       61 gcatctttta ctttcaccag cgtttatggg tgagcaaaaa caggaaggca aaatgccgca
      121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      241 aaaaataaac aaataggaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      841 caccgctggt agcggtgggt ttttttggtt tgcaagcagc agattacgcg cagaaaaaaa
      901 ggatctcaag aagatccttt tgatcttttc tacg
//
