LOCUS       sequence-18423-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM
  TITLE     Reproducible doxycycline-inducible transgene expression at specific 
            loci generated by Cre-recombinase mediated cassette exchange.
  JOURNAL   Nucleic Acids Res. 2005  . 33(17):e147.
  PUBMED    16204450
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(17):e147."
COMMENT     Sequence Label: pL3-TRE-LucGFP-2L
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(179..203)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(200..238)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(271..289,290..312,313..331,332..354,355..373,374..396,
                     397..415,416..438,439..457,458..480,481..499,500..522,
                     523..541)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    complement(572..605)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(638..654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(696..718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(912..931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcgccgggc ctttcttttg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccaagc ttgagctcga gatccccggg taccgagctc gaattcgggg ccgcggaggc
      121 tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga
      181 tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca cgcctacctc
      241 gacccgggta ccgagctcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      301 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      361 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      421 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      481 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      541 agtggtaaac tcgaggaagg ggttcatcga tataacttcg tataggagac tttatacgaa
      601 gttatctcga tgaaggggtt ctctagatcg agaattcact ggccgtcgtt ttacaaggcg
      661 cgcccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
      721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      841 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      901 tgcactctca gtacaatctg ctctgatgcc gcat
//
