LOCUS       sequence-184329-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MELK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MELK gRNA (BRDN0001145853)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        160..235
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    291..408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
       61 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      121 gtggaaagga cgaaacaccg ctgttacgca atcatcatcg ttttagagct agaaatagca
      181 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      241 taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      301 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      361 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      421 agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca aagatggata
      481 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
      541 ggaattggct ccggtgcccg tcagtgggca
//
