LOCUS       sequence-184331-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MELK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MELK gRNA (BRDN0001146713)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        162..237
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    293..410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
       61 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      121 tgtggaaagg acgaaacacc ggcagcacct gagttaatac agttttagag ctagaaatag
      181 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      241 tttaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa
      301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      421 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga
      481 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      541 tgggaattgg ctccggtgcc cgtcagtggg
//
