LOCUS       sequence-18449-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL
  TITLE     Regulation of the polarity protein Par6 by TGFbeta receptors 
            controls epithelial cell plasticity.
  JOURNAL   Science. 2005 Mar 11. 307(5715):1603-9.
  PUBMED    15761148
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Mar 11. 307(5715):1603-9."
COMMENT     Sequence Label: pCMV5-Flag-RhoA wt
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             90..662
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="AAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADI
                     EVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKH
                     FCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTK
                     DGVREVFEMATRAALQARRGKKKSGCLV"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattgat ctggtaccac gcgtatcgat accatggact
       61 acaaggacga cgatgacaag ggtcgacaag ctgccatccg gaagaaactg gtgattgttg
      121 gtgatggagc ctgtggaaag acatgcttgc tcatagtctt cagcaaggac cagttcccag
      181 aggtgtatgt gcccacagtg tttgagaact atgtggcaga tatcgaggtg gatggaaagc
      241 aggtagagtt ggctttgtgg gacacagctg ggcaggaaga ttatgatcgc ctgaggcccc
      301 tctcctaccc agataccgat gttatactga tgtgtttttc catcgacagc cctgatagtt
      361 tagaaaacat cccagaaaag tggaccccag aagtcaagca tttctgtccc aacgtgccca
      421 tcatcctggt tgggaataag aaggatcttc ggaatgatga gcacacaagg cgggagctag
      481 ccaagatgaa gcaggagccg gtgaaacctg aagaaggcag agatatggca aacaggattg
      541 gcgcttttgg gtacatggag tgttcagcaa agaccaaaga tggagtgaga gaggtttttg
      601 aaatggctac gagagctgct ctgcaagcta gacgtgggaa gaaaaaatct ggttgccttg
      661 tc
//
