LOCUS       sequence-184491-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human BLK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: BLK gRNA (BRDN0001146701)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..85
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        165..240
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    296..413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
       61 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      121 tcttgtggaa aggacgaaac accgcaggtc ccgatcattc ataggtttta gagctagaaa
      181 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      241 ttttttaagc ttggcgtaac tagatcttga gacaaatggc agtattcatc cacaatttta
      301 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      361 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      421 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg gtgcaaagat
      481 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
      541 gagtgggaat tggctccggt gcccgtcagt
//
