LOCUS       sequence-18450-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL
  TITLE     Regulation of the polarity protein Par6 by TGFbeta receptors 
            controls epithelial cell plasticity.
  JOURNAL   Science. 2005 Mar 11. 307(5715):1603-9.
  PUBMED    15761148
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Mar 11. 307(5715):1603-9."
COMMENT     Sequence Label: pCMV5-Flag-RhoA K6,7R
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             91..669
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="AAIRRRLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADI
                     EVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKH
                     FCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTK
                     DGVREVFEMATRAALQARRGKKKSGCLVL"
ORIGIN
        1 agagctcgtt ttagtgaccg tcagaattga tctggtacca cgcgtatcga taccatggac
       61 tacaaggacg acgatgacaa gggtcgacaa gctgccatcc ggcgccggct ggtgattgtt
      121 ggtgatggag cctgtggaaa gacatgcttg ctcatagtct tcagcaagga ccagttccca
      181 gaggtgtatg tgcccacagt gtttgagaac tatgtggcag atatcgaggt ggatggaaag
      241 caggtagagt tggctttgtg ggacacagct gggcaggaag attatgatcg cctgaggccc
      301 ctctcctacc cagataccga tgttatactg atgtgttttt ccatcgacag ccctgatagt
      361 ttagaaaaca tcccagaaaa gtggacccca gaagtcaagc atttctgtcc caacgtgccc
      421 atcatcctgg ttgggaataa gaaggatctt cggaatgatg agcacacaag gcgggagcta
      481 gccaagatga agcaggagcc ggtgaaacct gaagaaggca gagatatggc aaacaggatt
      541 ggcgcttttg ggtacatgga gtgttcagca aagaccaaag atggagtgag agaggttttt
      601 gaaatggcta cgagagctgc tctgcaagct agacgtggga agaaaaaatc tggttgcctt
      661 gtcttgtaat ctagacccgg gtggcatccc tgtgacccct ccccagtgcc tctcctgg
//
