LOCUS       sequence-184549-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human HK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: HK3 gRNA (BRDN0001146645)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        194..269
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    325..442
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
       61 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      121 cttgtggaaa ggacgaaaca ccgatcatgg ttcaaccgga atggtctcac accgcggttc
      181 tctgcgtgaa ggggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      241 aacttgaaaa agtggcaccg agtcggtgct tttttaagct tggcgtaact agatcttgag
      301 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
      361 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      421 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
      481 ccggctcgag ggggcccggg tgcaaagatg gataaagttt taaacagaga ggaatctttg
      541 cagctaatgg accttctagg tcttgaaagg
//
