LOCUS       sequence-184561-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TKFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TKFC gRNA (BRDN0001148963)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        164..239
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    295..412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
       61 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      121 cttgtggaaa ggacgaaaca ccgcagggag cggacggtag cgtgttttag agctagaaat
      181 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      241 tttttaagct tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa
      301 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      361 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      421 ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg tgcaaagatg
      481 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
      541 agtgggaatt ggctccggtg cccgtcagtg
//
