LOCUS       sequence-18467-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVU-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(218..237)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtacccggg gatcctctag agtcgacctg
       61 cagcccaagc ttcgtctaac aaaaaagcca aaaacggcca gaatttagcg gacaatttac
      121 tagtctaaca ctgaaaatta catattgacc caaatgatta catttcaaaa ggtgcctaaa
      181 aaacttcaca aaacacactc gccaaccccg agcgcatagt tcaaaaccgg agcttcagct
      241 acttaagaag ataggtacat aaaaccgacc aaagaaactg acgcctcact tatccctccc
      301 ctcaccagag gtccggcgcc tgtcgattca ggagagccta ccctaggccc gaaccctgcg
      361 tcctgcgacg gagaaaagcc taccgcacac ctaccggcag gtggccccac cctgcattat
      421 aagccaacag aacgggtgac gtcacgacac gacgagggcg cgcgctccca aaggtacggg
      481 tgcactgccc aacggcaccg ccataactgc cgcccccgca acagacgaca aaccgagttc
      541 tccagtcagt gacaaacttc acgtcagggt ccccagatgg tgccccagcc catctcaccc
      601 gaataatagc tttcccgcat taacgaacgc ctcaagacct tgggttcttg ccgcccacca
      661 tgccccccac cttgtt
//
