LOCUS       sequence-18473-e         365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPRT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     55..71
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     56..72
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(117..136)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          119..348
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 tgctcaccat ggtggcgacc ggtagcgcta ggacgcgtaa gcttaggcct ggattcgagg
       61 tcgacggtat cgataagctt gatatcgaat tcctgcagtt ggacctggga gtggacacct
      121 gtggagagaa aggcaaagtg gatgtcattg tcactcaagt gtatggccag atctcaagcc
      181 tgccacacct caagcttgac aacaaaaaga ttgtcttttc tgaccagatg gacgcggcca
      241 ccctcaaagg catcaccgcg ggccaggtga atatcaaatc ctcctcgttt ttggaaactg
      301 acaatcttag cgcagaagtc atgcccgctt ttgagaggga gtactcaccc caacagctgg
      361 ccctc
//
