LOCUS       sequence-18476-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin 
            promoter) AND p53 shRNA (H1 promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUTHshp53-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(213..231,232..254,255..273,274..296,297..315,316..338,
                     339..357,358..380,381..399,400..422,423..441,442..464,
                     465..483)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     603..619
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     604..620
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             729..740
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagatttcac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
       61 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
      121 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
      181 agcaaaaagc agatcgatcc gactttcact tttctctatc actgataggg agtggtaaac
      241 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      301 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      361 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      421 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      481 ggagtggtaa actcgagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
      541 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
      601 actcgaggtc gacggtatcg atgcggggag gcggcccaaa gggagatccg actcgtctga
      661 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
      721 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
      781 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
      841 tcagtgcccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
      901 cgccgtggca atagggaggg gggaaagcga aagtcccgga aaggagctga cagg
//
