LOCUS       sequence-18477-e         238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin 
            promoter) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUTHshGATA1-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtatgaga ccacagatcc ccgaagcgcc tgattgtcag tttcaagaga acagacaatc
       61 aggcgcttct ttttggaaaa gcttatcgat accgtcgcat gggaataact tcgtatagca
      121 tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat
      181 gtgagcctgg gagctctctg gctaactagg gaagccagtg cttaagcctc aataaagc
//
