LOCUS       sequence-18479-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB-2SM2
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(75..77,78..80,81..791)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(75..77,78..80,81..791)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(120..141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(381..400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     728..749
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatctccgg gcctttcgac ctctagcggg atccaggcct aagcttacgc gtcctagcgc
       61 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      241 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      541 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      661 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      781 agctgtacaa gtccggactc agatctcgac tagctagtag ctagctagct agtcgagctc
      841 aagcttcgaa ttcccgggct gca
//
