LOCUS       sequence-18480-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB-2SM2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcnagnncca gaggtcccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
       61 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      121 tcctttgaaa aacacgatga taataccatg gctagactgg acaagagcaa agtcataaac
      181 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
      241 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
      301 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
      361 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
      421 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
      481 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
      541 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
      601 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
      661 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
      721 ctaatcncta ntggnnnnnn nntagnnnnn nanntgtnaa agtgggtcnc caaaaaanaa
      781 aanaangtcg acgcggngng tgctttgtct cctcagcac
//
