LOCUS       sequence-18482-e         343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for shp53 (hUbiquitin 
            promoter) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUHshp53-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..148)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cttataagtt ctgtatgaga ccacagatcc ccgactccag tggtaatcta cttcaagaga
       61 gtagattacc actggagtct ttttggaaaa gcttatcgat accgtcgcat gggaataact
      121 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
      181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctc
//
