LOCUS       sequence-18484-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(204..223)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
       61 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      121 tgataatacc atggctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
      181 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc ttggtgtaga
      241 gcagcctaca ctgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
      301 tgagatgtta gataggcacc atactcactt ttgcccttta aaaggggaaa gctggcaaga
      361 ttttttacgc aataacgcta aaagttttag atgtgcttta ctaagtcatc gcaatggagc
      421 aaaagtacat tcagatacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
      481 agccttttta tgccaacaag gtttttcact agagaacgcg ttatatgcac tcagcgctgt
      541 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
      601 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
      661 tgatcaccag ggtgcagagc cagccttctt attcggcctt gaattg
//
