LOCUS       sequence-18492-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Mondragon A, Griffith EC, Sun L, Xiong F, Armstrong C, Liu JO
  TITLE     Overexpression and purification of human calcineurin alpha from 
            Escherichia coli and assessment of catalytic functions of residues 
            surrounding the binuclear metal center.
  JOURNAL   Biochemistry. 1997 Apr 22. 36(16):4934-42.
  PUBMED    9125515
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 1997 Apr 22. 36(16):4934-42."
COMMENT     Sequence Label: pET15b CnA CnB
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgtccgag cccaaggcaa ttgatcccaa
      121 gttgtcgacg accgacaggg tggtgaaagc tgttccattt cctccaagtc accggcttac
      181 agcaaaagaa gtgtttgata atgatggaaa acctcgtgtg gatatcttaa aggcgcatct
      241 tatgaaggag ggaaggctgg aagagagtgt tgcattgaga ataataacag agggtgcatc
      301 aattcttcga caggaaaaaa atttgctgga tattgatgcg ccagtcactg tttgtgggga
      361 cattcatgga caattctttg atttgatgaa gctctttgaa gtcgggggat ctcctgccaa
      421 cactcgctac ctcttcttag gggactatgt tgacagaggg tacttcagta ttgaatgtgt
      481 gctgtatttg tgggccttga aaattctcta ccccaaaaca ctgtttttac ttcgtggaaa
      541 tcatgaatgt agacatctaa cagagtattt cacatttaaa acagaatgta aaataaagta
      601 ttcagaacgc gtatatgatg cctgtatgga tgcctttgac tgccttcccc tggctgccct
      661 gatgaacaac agttcctgtg tgtgcatggt ggtttgtc
//
